ribo y deoxyribonucleosides  (Cytiva Europe)

Journal: Pharmaceuticals

Article Title: The Bioactive Compounds of Epimedium and Their Potential Mechanism of Action in Treating Osteoporosis: A Network Pharmacology and Experimental Validation Study

doi: 10.3390/ph17060706

Figure Lengend Snippet: Relative protein expression levels of HIF-1α and COL1A1 during MC3T3-E1 cell osteogenic differentiation under hypoxic conditions. ( A ) Western blot results. ( B ) Immunofluorescence results. Blue fluorescence represents cell nuclei, while green fluorescence represents HIF-1α. The blank group was cultured in α-MEM medium. The blank group was treated by normal medium; the control group was cultured in induction medium; the hypoxia group was treated by osteogenic medium with 100 μM CoCl 2 , set as the negative group in hypoxic condition; the 2″-O-RhamnosylIcariside II group was treated by osteogenic medium with 100 μM CoCl 2 and 6.25 μM 2″-O-RhamnosylIcariside II; and the Estradiol group was treated by osteogenic medium with 100 μM CoCl 2 and 10 μM Estradiol. Scar bar is 50 μm. Data are presented as mean ± SEM (n = 3). # p < 0.05 indicates statistical significance compared to blank group, * p < 0.05, *** p < 0.001, **** p < 0.0001 indicate statistical significance compared to hypoxia group.

Article Snippet: Fetal bovine serum from Australia was obtained from Avantor (Radnor, PA, USA, Cat#76294-180). α-MEM medium was purchased from Cytiva (Logan, UT, USA, Cat# SH30265.01).

Techniques: Expressing, Western Blot, Immunofluorescence, Fluorescence, Cell Culture, Control